USE+OF+ENERGY+&+METABOLISM

Casey Rodenberg Brielle Soluri Rachel Mollozzi Lucia Garabo

What characteristic of living things is observed in this investigation?
 * __Purpose/Problem:__**


 * __Hypothesis:__** If unknowns mixed with sugar and water produce a gas product, then they are considered living and using energy.

1. Sugar 2. Warm water 3. FIve test tubes 4. Test tub rack 5. Three unknown substances 6. Bromothymol Blue 7. Four Balloons 8. Glucose test strips 9. Scale
 * __Materials:__**


 * Control:** sugar.
 * Independent Variable:** Unknown's A, B, & C.
 * Dependent Variable:** the amount of energy gained

__**Procedure:**__ 1. Obtain and label four test tubes with the following: a. Test tube A a. Test tube B b. Test tube C b. Test tube CONTROL

2. Add to each test tube 25 ml of warm water. Add water only three fourths of the way up. a. Test tube A b. Test tube B c. Test tube C d. Test tube CONTROL

3. Gently shake the contents of each test tube. Make sure none of the materials remains clumped at the bottom.

4. Dip a glucose test strip into each of the four test tubes and record in a table below:

5. Slip a balloon over the mouth of each test tube.

6. Sometime during the day but at least thirty minutes after completing setup record in the table below:

7. Write the time of the initial setup

8. After twenty four hours record your observations of the balloon and the contents in the table below:

9. Fill a 5th test tube with bromothymol blue.

10. Carefully remove the ballon that has inflated the most making sure to release contents of balloon.

11. Slip the balloon over the mouth of the test tube with bromothymol blue.

12. Squeeze contents out of balloon into test tube.

13. Gently mix test tube and observe and record any color changes.

14. Test the four test tubes with the glucose test strip and record.

Glucose Testing
 * Time || Unknown A || Unknown B || Unknown C || Control ||
 * Initial || 100 || 100 || 500 || 250 ||
 * Minutes 180 || 100 || 250 || 1000 || 500 ||
 * 24 hours || 100 || 250 || 1000 || 500 ||

__**Bromothymol Blue:**__

Test Tube C - turned blue to green to yellow.

Balloon Inflation
 * Time || Unknown A || Unknown B || Unknown C || Control ||
 * Initial || none || none || very inflated || none ||
 * 24 hours || none || none || inflated || none ||